The DNA Sequencing Facility was created by the merger of the Vanderbilt Ingram Cancer Center DNA core with the Division of Genetic Medicine's sequencing operations. The facility has been under the direction of Alfred L. George, Jr. M.D. since May 2002. The purpose of the DNA Sequencing Facility is to provide academic researchers within and outside the Vanderbilt community high quality DNA sequencing at minimal cost.
The DNA Sequencing Facility uses state-of-the-art technology and methodology to produce high quality DNA sequences in a time span of 1-2 business days. DNA samples are all sequenced using BigDye Terminator chemistry and resolved on the ABI 3730xl DNA Analyzer. BigDye Terminator chemistry utilizes ddNTPs that are labeled with a fluorescent dye specific for each nucleotide, allowing sequencing in one reaction tube. All sequencing reactions are set up robotically using Packard's Multiprobe II Liquid Handling Platform, and cycled on a high capacity thermal cycler (MJ Research Tetrad). The sequences are then run on the ABI 3730xl DNA Analyzer. The ABI 3730xl uses a capillary electrophoresis system that creates a sensitive detection system, long sequence reads (up to a 1000 bases for high quality DNA), short run times, and low operating/reagent costs. The ABI 3730xl DNA Analyzer is an automated system (sample loading, separation matrix preparation, and sequence analysis) which coupled with the facility's liquid handling robot, dramatically reduces the introduction of human error.
FEATURES:
|
|
