Too Many Peaks

Possible Causes:

1. Mixed Plasmid Templates: This might happen, for example, when accidentally picking more than one colony for plasmid amplification. Solution: Re-streak the bacteria and pick well-isolated colonies.

2. Sample Contamination: Sample contamination can occur at many points during DNA preparation and handling. Solutions: Prepare a fresh aliquot of DNA template for sequencing.

3. Non specific Primer: A primer that binds the DNA template non-specifically can initiate the sequence reaction from multiple sites along the template. Solution: Try using Primer Design software to avoid false priming sites.