PRO-ACTIVE REDO POLICY

We strive to obtain the best quality sequence for each and every sample. To remedy problems caused by random reaction failures or inherent sample problems, most poor quality reactions are repeated once at no cost to the investigator. During the examination of the initial sequencing reaction result, the facility categorizes each failure. Each failure category has a set procedure that is utilized in the second sequencing reaction in order to hopefully correct the initial failure. For example, when a sample is of poor quality due to having a low signal (minimal sequence production), the facility will increase the amount of DNA added in the sequencing reaction.

Samples that do not produce any detectable sequence signal will not automatically be re sequenced. The researcher will be notified the reason the reaction failed and can request the facility to re sequence the sample. If the researcher wants the facility to re sequence the sample the researcher will be charged for the re sequencing if the reaction fails again. If the re sequencing is successful, the facility will pay for the re-sequencing of the sample. This policy was implemented following a 6 month study where it was found that 90% of the samples that did not produce a detectable sequence signal in the initial sequencing reaction failed during the re sequencing reaction.

We will continue to repeat other types of failed reactions at no additional charge.

All redo sequences that are above or below the facility’s quality standard are returned to the investigator. It is the investigator’s disgression and cost to attempt to sequence a problem sample more than twice. The DNA Sequencing Facility will gladly help in troubleshooting any problem sequences or the investigator can review our Troubleshooting section.